It seems like every paper I look at these days has Nonnegative Matrix Factorization (NMF) in its methods somewhere. From machine learning, to calcium imaging, the seemingly magic ability of NMF to pull apart signals gets a lot of use. In this post I want to explain NMF to people who have zero understanding of linear algebra, show a few applications, and maybe give you some inspiration of how to use NMF in your own work.
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Matlab
Filtering – A practical guide
Finding good information on how filters work, what the different types of filters mean, and how you should filter your data is hard. Lots of explanations only make sense if you have a year or two of electrical engineering education, and most of the rest are just a list of rules of thumb. I want to try to get you to a place where you can test your own filter settings, and show you the importance of the rules of thumb without going into the relatively complex math that is often used to explain filters. Warning: a lot of the code I’m going to use requires the Matlab Signal Processing Toolbox. If you don’t have it, you wont be able to execute the code yourself, but hopefully you’ll still be able to follow along with the logic.
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Merging ROIs in suite2p
Suite2p is a wonderful Matlab toolbox written by Marius Pachitariu for analyzing population calcium imaging data. It uses a number of computational tricks to automate and accelerate the process (so no more drawing regions of interest (ROIs) by hand!). However, I spend most of my time imaging dendrites and axons, and here suite2p has a problem. Suite2p uses a heuristic that is looking for approximately elliptical ROIs, and hence it tends to split axons/dendrites into a large number smaller ROIs. The problem was simple: how can we merge the ROIs belonging to single cells? Well I used the logic that ROIs that belong to the same neuron should have highly correlated calcium signals (yes, I can imagine a situations where this wont be the case in dendrites, but bAPs will still dominate the calcium trace 99.9% of the time). Hence I simply correlate each ROI with every other ROI. ROIs with a correlation coefficient above some user settable threshold are considered to be part of the same process.
The main script is available here, and it requires distinguishable_colors.m (which in turn requires the image processing toolbox I believe).
The code is relatively well documented/commented, and there is even a ‘Help!’ button. If anyone has any problems with it, please let me know.
Neuronal Modelling – The very basics. Part 1.
I think a lot of people are confused about neuronal modelling. I think a lot of people think it is more complex than it is. I think too many people think you have to be a mathematical or computational wizard to understand it, and I think that leads to a lot of good modelling being discounted and a lot of bad modelling being let through peer-review. I’m here to tell you that biophysical models on neurons don’t have to be hard to implement, or understand. I’m going to start you off on the ground floor, in fact, below the ground floor, this is the basement level. All you need to know is a little coding (I’m going to do both Matlab and Python to start). But I should temper your expectations. When we are done, you’re not going to be ready to publish fully fledged multi-compartment models of neurons, but at least you will understand the fundamental principles of what is happening. And the most fundamental of all is this…
A cheap LoFi speaker – Impedance II
I needed to make a little speaker. The real reason? Because of a retro gaming session at work. However, there have been several times that I’ve needed a small cheap speaker that was suitable for delivering simple stimuli. I thought I would post a schematic and take this opportunity to give you an example of how Matlab can make impedance calculations easier, something I’ve talked a little about before.
Impedance: A beginner’s guide -or- how I learned to stop worrying and let Matlab do the work.
Impedance is a concept that some people say they understand, but deep in their heart of hearts they know they don’t. I’m going to try and help you get a more helpful conceptual understanding of it. But to try and entice you into learning about it, I’m going to pose you a question: where does a sine wave go, when it’s amplitude is zero?
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